Kev Txiav Txim Method Ntawm Fungicide

Cov tshuaj tua kab mob lossis cov kab mob bactericidal ntawm tus neeg sawv cev feem ntau tau hais tias yog "thaj chaw nruab nrab koob tshuaj", uas yog, koob tshuaj (qhov nruab nrab tus neeg tuag, mg / kg) yuav tsum tua ib nrab (50 feem pua) ntawm cov neeg lom neeg feem ntau yog abbreviated li LD50. Yog hais tias qhov concentration sawv cev rau qhov koob tshuaj, nws yog "Lethal Medium Concentration", abbreviated li LC50. Cov tshuaj fungicides tau qhia los ntawm ED50 lossis EC50, uas yog, koob tshuaj lossis cov ntsiab lus xav tau los tiv thaiv 50 feem pua ​​​​ntawm cov kab mob spores.
1. Spore Germination Assay
Txau cov kua tshuaj sib txawv rau ntawm qhov chaw ntawm cov iav slides los yog daim hlau tiaj tus, kom muaj nuj nqis ntxiv cov tshuaj tiv thaiv kab mob, thiab tom qab tiv tauj cov kua tshuaj, xyuas qhov feem pua ​​​​ntawm cov kab mob spores nyob rau hauv lub tshuab tsom iav tom qab qee lub sijhawm incubation.
2. Thaj tsam ntawm txoj kev inhibition
Sib tov qhov ncua sij hawm ntawm cov kab mob pathogenic spores lossis hyphae nrog agar nruab nrab, tom qab condensation, tso cov ntawv lim dej tsis muaj kab mob (kwv yees li 6 hli inch) dipped nyob rau hauv ntau qhov sib txawv ntawm cov tshuaj daws teeb meem ntawm qhov nruab nrab, thiab coj lawv ntawm qhov kub thiab txias. rau ib lub sij hawm. Tom qab ib lub sij hawm ntawm lub sij hawm, vim lub diffusion ntawm cov kua tshuaj, qhov kev loj hlob ntawm cov kab mob yog inhibited, uas yog, ib tug "inhibition cheeb tsam" yog tsim. Ntsuas qhov loj ntawm thaj tsam ntawm inhibition los sib piv cov kab mob fungicides.
3. Kev Ntsuam Xyuas Kev Loj Hlob
Ntxiv cov tshuaj tov rau agar nruab nrab, inoculate cov kab mob tom qab condensation, soj ntsuam kev loj hlob ntawm cov colony tom qab 24-48 teev, xam qhov kev loj hlob tus nqi, thiab muab piv nrog rau kev loj hlob tus nqi ntawm cov pab pawg neeg tswj tsis muaj cov tshuaj.